Quantification of human skin diffusivity during in vivo optical clearing
Daria K. Tuchina,1,2 Polina A. Timoshina,1,2 Valery V. Tuchin1,2,3
1Institute of Physics and Science Medical Center, Saratov State University, Saratov, Russia
2Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, Tomsk, Russia
3Institute of Precision Mechanics and Control, FRS “Saratov Scientific Centre of the RAS”, Saratov, Russia
Abstract
Diffuse reflectance spectra of the human skin in vivo was measured during the optical clearing by using such optical clearing agents (OCAs) as aqueous 40% glucose, 60% and 70% glycerol solution, and aqueous-alcoholic 50% fructose solution. Spectra were recorded in the visible and NIR spectral ranges using a USB4000-Vis-NIR multichannel spectrometer (Ocean Optics, USA) at wavelengths of 350 - 1000 nm.
At OCA topical application to the human skin the spectra and time dependencies of diffuse reflection of the skin showed a decrease caused by optical clearing of the skin upper layers. The characteristic diffusion time, the effective diffusion coefficient of agents in the skin, and the effective skin permeability coefficient for OCA components and interstitial water were estimated from the obtained spectra. Aqueous 70% glycerol solution showed the fastest effective diffusion coefficient of (3.3±0.4)×10−6 cm2/s.
This work was supported by the Russian Science Foundation grant No. 23-14-00287.
Speaker
Daria K. Tuchina
Saratov State University
Russian Federation
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Discussion
Andrey Veniaminov
Does the technique allow for revealing spatial heterogeneity of tissue (skin)?
Does the technique's applicability depend on the skin depth?
Daria K. Tuchina
Dear Professor Veniaminov,
Thank you for your question.
OCT is probably more suitable for detecting spatial heterogeneity of tissue, however, the measured spectra show that the tissue contains components that interact with light differently, i.e. absorb light at different wavelengthsб it means it is heterogeneous.
Yes, the technique depends on the depth of tissue probing, since light spreads differently within the different thicknesses of the region.
If you have any other questions, we will try to answer them.
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