Structural changes in skin and skeletal thigh muscle of the rat in experimental diabetes
Ekaterina N. Lazareva1,2,3 Alla B. Bucharskaya1,3, Andrey M. Zakharevich1, Nikita A. Navolokin1,3, Natalya A. Shushunova1,3, Valery V. Tuchin1,2,3; 1Saratov State University, Saratov, Russia;2Tomsk State University, Tomsk, Russia; 3Saratov State Medical University, Saratov, Russia; 4Institute of Precision Mechanics and Control RAS, Saratov, Russia
Abstract
The importance of studying structural changes in skeletal muscles during the development of diabetes is clear, due to the fact that muscles are responsible for the majority of insulin-stimulated whole body glucose removal in healthy patients.The present study reports the application of scanning electron microscopy (Mira II LMU microscope,TESCAN, Czech Republic) to determine structural changes in alloxan - induced diabetes in rat skin and skeletal muscles, which emphasizes the promising potential of this method in medical research. Tissue sample preparation was conducted by standart histologic methods. The results showed that diabetes induces a number of changes in skin and thigh muscle structure, which was confirmed by histologic examination. In particular, a decrease in the integrity of collagen molecules was found in muscle during the development of experimental diabetes. The severity of structural changes in skin and muscle depended on glucose levels in the experimental diabetes model.
This work was supported by grant of the Russian Science Foundation # 24-44-00082
Speaker
Ekaterina N. Lazareva
Scientific and Educational Institute of Optics and Biophotonics, Saratov National Research State University named after N.G. Chernyshevsky (410012 Saratov, Astrakhanskaya st. 83, Russia)
Russia
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