Assessing Glycerol Diffusion Properties in the Pancreas
Maria R. Pinheiro1,2, Maria I. Carvalho1,2, Valery V. Tuchin3,4,5, Luís M. Oliveira1,6; 1Institute for Systems and Computer Engineering, Technology and Science (INESC TEC), Campus da FEUP, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; 2Department of Electrical and Computer Engineering, Faculty of Engineering of Porto University (FEUP), Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; 3Institute of Physics and Science Medical Center, Saratov State University, 83 Astrakhanskaya str., Saratov 410012, Russian Federation; 4Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control of the FRC “Saratov Scientific Centre of the Russian Academy of Sciences,” 24 Rabochaya, Saratov 410028, Russian Federation; 5Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, Tomsk 634050, Russian Federation; 6Physics Department, School of Engineering, Polytechnic of Porto, Rua Dr. António Bernardino de Almeida nº 431, 4249-015 Porto, Portugal
Abstract
The optical immersion clearing technique enhances tissue transparency through the reduction of light scattering. To characterize the mechanisms behind optical clearing (OC) treatments, the analysis of agent and water diffusion within the tissue is essential. Tissue dehydration and refractive index (RI) matching are the main OC mechanisms, being directly associated with an outward flow of mobile water from the tissue, and with an inward flow of the OC agent into the tissue, respectively. The use of highly concentrated solutions in OC treatments might also lead to a third mechanism, designated as protein dissociation, which can induce high-efficiency transparency effects in the ultraviolet spectral range. The present study was conducted to evaluate the diffusion properties of glycerol and water in the pancreas and characterize the two main OC mechanisms. Performing kinetic collimated transmittance (Tc) measurements at various wavelengths during treatments with water-glycerol solutions containing different osmolarities, it was possible to estimate the characteristic diffusion time (τ) for glycerol in pancreas as 220.8s, and also the τ for water in pancreas as 59.1s. Performing also kinetic thickness measurements during the treatments where the glycerol and the water fluxes are unique, the characteristic diffusion coefficients (D) for the water and glycerol in the pancreas were calculated as 2.11 x 10-6 cm2/s and 6.26 x 10-7 cm2/s, respectively. This detailed understanding of diffusion properties aids in optimizing OC treatments, enhancing tissue transparency for better diagnostic and therapeutic outcomes.
Link to file with lecture
https://disk.yandex.ru/i/TPlm5lFi9dkmlA
Speaker
Maria Rosário Pinheiro
Institute for Systems and Computer Engineering, Technology and Science (INESC TEC), Porto, Portugal
Portugal
Report
File with report
Discussion
Ask question
