Determination of ciprofloxacin concentration in aqueous soil extracts in the presence of humic substances using synchronous fluorescence spectra

Abstract

The antibiotic ciprofloxacin is widely used in medicine, agriculture and veterinary.
Accidental releases of antibiotics into wastewater, soil, or natural water reservoirs
are common occurrences that fundamentally affect natural ecosystems. That is why
it is extremely important to develop methods for determining the concentration of
antibiotics in water and soil, taking into account the presence of organic substances
of natural origin (humic substances) in them. This work is aimed to develop an
optical method for determining the concentration of ciprofloxacin in the presence
of humic substances in aqueous medium. Ciprofloxacin exhibits fluorescence with
an emission maximum at 435 nm and an excitation maximum at 275 nm, however,
its emission band in natural water or soil overlaps with the fluorescence of humic
substances. To determine the concentration of ciprofloxacin in the presence of
humic substances, we propose to use synchronous fluorescence spectra that
selectively highlight the fluorescence of the antibiotic. It was found that
ciprofloxacin is most clearly detected at a difference in excitation and emission
wavelengths of 160 nm. Quantitative determination of ciprofloxacin is carried out
by the wavelength-integrated intensity of the spectrum, obtained as the difference
between the synchronous fluorescence spectrum of the soil extract with and
without ciprofloxacin. In a model experiment with 6 types of soil contaminated
with ciprofloxacin, the antibiotic concentrations in soil extracts were measured
with the help of developed technique.

Speaker

Tsvetkova Anastasia Denisovna
Faculty of Physics, M.V.Lomonosov Moscow State University, Moscow
Russia

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