Unique algorithm, data processing and physical laws for the evaluation of embryo photon emission and viability
József Bódis1.,4.,7., József Berke1.,2., Zoltán Bognár1.,3.,4., István Gulyás1, Dávid Berke5., Attila Enyedi2., Veronika Kozma-Bognár2.,6., Péter Mauchart1.7., Bernadett Nagy*1.,4.,7., Ákos Várnagy1.,4.,7., Kálmán Kovács1.,4.,7; 1National Laboratory on Human Reproduction, University of Pécs, Pécs, Hungary; 2Dennis Gabor University, Department of Drone Technology and Image Processing, Budapest, Hungary; 3Department of Medical Biology and Central Electron Microscope Laboratory, Medical School, University of Pecs, Pécs, Hungary; 4HUN-REN–PTE Human Reproduction Scientific Research Group, Pécs, Hungary; 5John von Neumann Computer Society, Multimedia in Education Section, Budapest, Hungary; 6Dennis Gabor University, Rector’s cabinet, Budapest, Hungary
7Department of Obstetrics and Gynecology, Medical Scholl, University of Pecs, Pécs, Hungary
Abstract
Living cells exhibit ultra-weak photon emission, which originates from metabolic reactions related to physiological processes. Photon emission of cells provides an opportunity to monitor physiological processes. Photon emission studies carried out with living cells did not detect spontaneous photon emission, but measured photon emission induced by some kind of stimulation!
In vitro fertilization (IVF) resulted in more than 9 million children since 1978, and due to the social changes taking place today, it is of increasing importance. During IVF more embryos are usually conceived than can be transfer. This is even more true with today's guidelines, which prefer the transfer of one or two embios. Several methods are used to select embryos suitable for transfer, none of which is perfect. The detection of spontaneous (non-induced!) photon emission of developing embryos can be used to make the selection of embryos suitable for transfer.
In order to understand the self-similar structure of the energy emitted by embryos, i.e. to evaluate the higher entropy energy data of embryos, we developed a new algorithm for calculating the entropy-weighted spectral fractal dimension. Based on this method, our research ensured that we could distinguish between live, degenerated, frozen and fresh concieved mouse embryos, and the background.
The novel detection of the ultra-weak photon emission of mouse embryos can be the basis for the construction of the photon emission embryo control system (PEECS). It is hypothesized that ultra-weak photon emission fingerprints of embryos can be used to select viable specimens in an ideal dark environment.
Literature
1. Cifra, M. & Pospisil, P. Ultra-weak photon emission from biological samples: definition, mechanisms, properties, detection, and applications. // J. Photochem. Photobiol. B Biol. 139, 2–10 (2014).
2. Beloussov, L. V., Burlakov, A. B. & Louchinskaia, N. N. Biophotonic pattern of optical interaction between fish eggs and embryos. // Indian J. Exp. Biol. 41, 424–430 (2003).
3. Berke, J. – Gulyás, I. – Bognár, Z. – Berke, D. – Enyedi, A. – Kozma-Bognár, V. - Mauchart, P. – Nagy, B. – Várnagy, Á. – Kovács, K. – Bódis, J. (2024): Unique algorithm for the evaluation of embryo photon emission and viability. // Scientific Reports 14, 15066 (2024).
https://doi.org/10.1038/s41598-024-61100-8.
4. Bódis, J. et al. How to reduce the potentially harmful effects of light on blastocyst development during IVF. // Med. Princ. Pract. 29 (6), 558–564 (2020).
5. Bognar, Z. et al. The effect of light exposure on the cleavage rate and implantation capacity of preimplantation murine embryos. // J. Reprod. Immunol. 132, 21–28 (2019).
Speaker
József Bódis1,2,3, József Berke1,4
1National Laboratory on Human Reproduction, University of Pécs, Pécs, Hungary; 2HUN-REN–PTE Human Reproduction Scientific Research Group, Pécs, Hungary; 3Department of Obstetrics and Gynecology, Medical Scholl, University of Pecs, Pécs, Hungary; 4Dennis Gabor University, Department of Drone Technology and Image Processing, Budapest, Hungary
Hungary
Discussion
Ask question
