Raman spectroscopy as a method to study the haemocompatibility of materials containing carbon nanostructures in a biopolymer matrix
Kristina D. Popovich1,2, Alexander Yu. Gerasimenko1,2; 1Institute of Biomedical Systems, National Research University of Electronic Technology, MIET, Zelenograd, Russia;
2Institute for Bionic Technologies and Engineering, I.M. Sechenov First Moscow State Medical University, Moscow, Russia
Abstract
The following solution to the problem of thrombosis when a medical device comes into contact with blood is proposed. It involves modifying the surface composition of the device in order to increase the haemocompatible properties of the material.
The objective of manufacturing haemocompatible coatings is to prevent the processes that lead to the formation of blood clots, particularly the initial adhesion of the albumin protein, which occurs in the initial seconds of contact between the foreign material and the blood. This adhesion is a stimulator of thrombosis.
The proposed method enables the determination of the adhesion of the albumin protein to a coating sample comprising carboxylated carbon nanotubes in a collagen matrix through Raman spectroscopy, thereby identifying the haemocompatible properties of in-vitro coatings. This method entails a comparison of the Raman spectra of composite coatings prior to and following contact with a bovine serum albumin solution, the structure of which is analogous to that of human serum albumin. The conclusions regarding the degree of haemocompatibility of the samples were formulated in relation to the results obtained for a control sample of titanium, which is commonly utilised as the material for a device in contact with blood. The presented method demonstrated a high degree of haemocompatibility of the developed coatings with respect to the control titanium.
The work was carried out as part of a major scientific project with financial support from the Russian Federation represented by the Ministry of Science and Higher Education of the Russian Federation under agreement No. 075-15-2024-555 dated April 25, 2024.
Speaker
Popovich Kristina
1Institute of Biomedical Systems, National Research University of Electronic Technology, MIET, Zelenograd, Russia; 2Institute for Bionic Technologies and Engineering, I.M. Sechenov First Moscow State Medical University, Moscow, Russia
Russia
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