Determination of the characteristics of complexes of native with glycated human serum albumin based on the results of polarization measurements of eosin - probe fluorescence
Vyacheslav I. Kochubey1, Alexander B. Pravdin1, Andrey G. Melnikov2, Denis A. Bykov2, Alexander R. Milkin2, Rustam D. Sadykov2
1Saratov State University, Saratov. Russia; 2Yuri Gagarin State Technical University of Saratov, Russia
Abstract
Annotation
Polarization measurements of the fluorescence of the probe – eosin bound to proteins made it possible to detect an increase in the anisotropy of eosin fluorescence during the transition from a solution of human serum albumin (HSA) and glycated albumin (gHSA) to a mixture containing HSA and gHSA. The calculations performed to determine the rotational correlation and the rotational diffusion rate constant under the assumption of the formation of complexes of the composition: HAS - gHSA, gave good agreement with the experimental results for determining the anisotropy of eosin fluorescence.
Studies conducted by methods of dynamic light scattering of solutions of HSA, gHSA and mixtures of HSA - gHSA experimentally confirmed the assumption of the formation of complexes: HSA - gHSA.
Thus, it can be assumed that a complex composition complex is involved in the process of rotational diffusion in a mixture of HSA and gHSA: probe - HSA - gHSA. The results obtained are in demand in the development of methods for the early diagnosis and course of a disease such as diabetes mellitus.
Speaker
Denis A. Bykov
Postgraduate student of the Department of Physics Yuri Gagarin State Technical University of Saratov
Russia
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