Synthesis and study of the photo‐ and sonodynamic properties of microbubbles functionalized with photodynamically active dye
Ksenia A. Parutina 1, Polina G. Rudakovskaya 1, Roman A. Barmin 1, Tatyana M. Estifeeva 1.
1Center for Photonic Science and Engineering, Skolkovo Institute of Science and Technology, Moscow, Russia
Abstract
This paper presents the development and comprehensive analysis of microbubbles functionalized with photodynamically active dyes to investigate their photo- and sonodynamic properties. This topic is relevant in the context of developing new approaches in cancer treatment, as photodynamic therapy (PDT) and sonodynamic therapy (SDT) demonstrate significant potential for the selective destruction of cancer cells.
The work describes the synthesis of microbubbles with a protein-polymer shell using ultrasonic processing. Zinc phthalocyanine (ZnPc16−) and Photoditazine (PD) dyes are used as photosensitizing agents. The absorption spectra analysis allowed for the determination of optimal wavelengths for the activation of the photosensitizers. The viability assessment of MCF-7 cell lines using the Alamar Blue assay demonstrated high phototoxicity of microbubbles functionalized with Photoditazine under light exposure. Furthermore, a reduction in the concentration zone of PD was achieved, indicating the promise of the developed microbubbles for use in PDT. Additionally, the optimal ultrasound exposure mode for further in vitro studies was determined.
This work not only expands the possibilities of using photosensitizers in cancer therapy but also proposes new ways to improve the efficiency and safety of treatment. Special attention is given to the selection of conditions for the synthesis of microbubbles, which allows for maximizing their functional properties and minimizing side effects. Thus, the developed microbubbles have high potential for clinical application in PDT and SDT, providing new opportunities for combating cancer. The research conducted in this paper lays the groundwork for subsequent experimental and clinical trials aimed at improving photodynamic and sonodynamic therapy methods.
Speaker
Ksenia A. Parutina
Center for Photonic Science and Engineering, Skolkovo Institute of Science and Technology, Moscow, Russia
Russia
Discussion
Ask question
