Approach to evaluate the efficacy of ex vivo root canal treatment using microtomography
E.V. Sadyrin1, E.V. Laktionov2; 1Don State Technical University, Rostov-on-Don, Russia; 2State budgetary institution of the Rostov region “Dental clinic” in Rostov-on-Don, Rostov-on-Don, Russia
Abstract
This paper presents a new approach to assessing the effectiveness of root canal treatment using rotary dental instruments (files) in ex vivo conditions, which is based on computed X-ray microtomography methods. This approach includes a detailed comparison of three-dimensional tooth models before and after the endodontic procedure. During the study, the obtained data are segmented, and the root canal volume is calculated using specialized software developed in accordance with a specific protocol. Additionally, this approach analyzes the microgeometry of the root canals, which allows for a deeper understanding of their structural characteristics. As an illustration of the application of the proposed method, the study considers the features of root canal instrumentation in human two- and three-root molars, which emphasizes the importance and relevance of this approach in the field of endodontics. Procedure was performed by a practicing dentist using the ProTaper Next file system (Maillefer, Ecublanc, Switzerland) in accordance with the manufacturer's recommended protocol. Microtomography of both samples was carried out using an Xradia Versa 520 гтше (Carl Zeiss Xray Microscopy, Inc., Pleasanton, USA) in cylindrical polymer holders with distilled water with the same parameters: X-ray tube voltage 110 kV, power 9.5 V, pixel size 14.7 μm, rotation sample at 360°, exposure time 1 s, filter on X-ray tube HE6. Two human molars (two- and three-rooted ones) were removed from patients for orthodontic indications at the State budgetary institution of the Rostov region “Dental clinic” in Rostov-on-Don. The local independent ethical committee of Don State Technical University approved the study (conclusion No. 1 of January 15, 2024), patients provided informed consent. The work was carried out with the financial support of the Russian Science Foundation, grant No. 22-19-00732. The results were obtained using the resources of the Resource Center of Collective Usage “Materials” of Don State Technical University.
Speaker
Evgeniy V. Sadyrin
Don State Technical University
Russian Federation
Discussion
Ask question
