Nanointerfaces based on carbon framework nanomaterials for nerve tissue cells stimulation
Uliana E. Kurilova1,2, Denis T. Murashko2, Irina A. Suetina3, Leonid I. Russu3, Marina V. Mezentseva3, Artem V. Kuksin2, Victoria V. Suchkova1,2, Alexander Yu. Gerasimenko1,2;
1 I.M. Sechenov First Moscow State Medical University, Moscow, Russia;
2 National Research University of Electronic Technology, Moscow, Zelenograd, Russia;
3 National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation, Moscow, Russia
Abstract
This work presents the results of physical and biological studies of nanointerfaces made with carbon materials of three compositions: carbon nanotubes, reduced graphene oxide and hybrid structures from their combination. Nanomaterials were formed by spray deposition and laser structuring of layers, the topology was formed on the surface of the samples using laser radiation from a pulsed ytterbium laser. According to scanning electron microscopy and Raman spectroscopy, it was found that the formation of a conductive network of nanomaterials occurs during laser irradiation with power of 0.07 W. At the same time, the electrical conductivity of the samples increases, the maximum value was obtained for the sample of hybrid structures. Electrical stimulation of cells on the surface of the samples was carried out using the developed setup based on a culture plate. After 72 hours of cultivation, a significant excess of nerve tissue cells was found on samples with carbon nanotubes in the composition; for connective tissue cells, the best results were shown for the sample based on graphene oxide. A greater number of cells is observed on parts of the sample with laser structuring compared to areas without structuring. Thus, the obtained nanointerfaces can be successfully used to transmit electrical signals in various bioelectronic devices.
The work was carried out as part of a major scientific project with financial support from the Russian Federation represented by the Ministry of Science and Higher Education of the Russian Federation under agreement No. 075-15-2024-555 dated April 25, 2024.
Speaker
Uliana E. Kurilova
I.M. Sechenov First Moscow State Medical University, National Research University of Electronic Technology
Russia
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