Analysis of microobject interaction forces in electric fields using optical tweezers on human peripheral blood lymphocytes
Maksim A. Dragun1, Daniil A. Bystrov1, Ivan A. Kushnir1, Anna A. Kopylova1, Egor V. Yakovlev1, Sofia A. Korsakova1, Alla A. Shabalina2, Maksim N. Andreev2, Alla B.
Salmina1,2, Sergey N. Illarioshkin2, and Stanislav O. Yurchenko1;
1 - Center “Soft Matter and Physics of Fluids”, Bauman Moscow State Technical University, Moscow, Russia;
2 - Research Center of Neurology, Moscow, Russia
Abstract
The direct of interaction forces between microobjects subjected to an external anisotropic alternating electric field is of substantial fundamental and practical significance. The combination of alternating electric fields and optical trapping techniques allows for the investigation of the intrinsic properties of microobjects, including biological entities. It is well established that the dielectric properties of mammalian cells are influenced by various pathological and physiological conditions, particularly those involving alterations in the cell membrane and changes within intracellular phases, such as organelles, biomacromolecules, and fluid compartments. It can be reasonably inferred, therefore, that the dielectric characteristics of peripheral blood cells may be notably altered in conditions associated with intracellular protein aggregation, such as neurodegenerative diseases and the process of aging. This study introduces an experimental setup designed to measure interaction forces between microobjects, specifically freshly isolated peripheral blood lymphocytes, in the presence of an external electric field. This configuration integrates optical tweezers with an electrode cell. Initial measurements conducted on human peripheral blood lymphocytes have demonstrated a strong correlation between experimental results and theoretical predictions. Due to its technological versatility, this configuration is well-suited for investigating fundamental phenomena in classical liquids, solids, and biological systems, making it relevant for researchers in soft matter physics, photonics, materials science, biomedicine, and tissue engineering.
The work is supported by a grant from the Ministry of Science and Higher Education of the Russian Federation for major scientific projects in priority areas of scientific and technological development (project # 075-15-2024-638).
Speaker
Maksim A. Dragun
Bauman Moscow State Technical University
Russia
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