Optical clearing of human skin in vivo using an aqueous solution of glucosamine hydrochloride and molecular modeling of its interaction with collagen.
K. V. Berezin1, E.V. Grabarchuk2, A. M. Lichter2, K. N. Dvoretski3, V. V. Tuchin 1,4; 1Institute of Physics, Saratov State University, Saratov, Russia, 2Astrakhan Tatishchev State University, Russia,, 3Saratov State Medical University, Russia, 4Science Medical Center, Saratov State University, Saratov, Russia
Abstract
Using the OCT method, the results of in vivo immersion optical clearing of human skin were obtained using an aqueous solution of glucosamine hydrochloride as an immersion agent. To assess the effectiveness of optical clearing, the values of the rate of change in the light scattering coefficient were determined, obtained using the average A-scan of the OCT signal in a dermal area with a depth of 350 to 700 µm. As a result of molecular modeling by quantum chemistry methods HF/STO3G/DFT/B3LYP/6 311G(d) of the interaction of molecules of a number of immersion clearing agents (glucosamine, galactosamine, 1-deoxynojirimycin, sucrose, iohexol, ribose, fructose, glucose, glycerol) with a mimetic peptide Collagen (GPH)3 correlations were established between the efficiency of optical clearing and the energy of intermolecular interaction of clearing agents with a collagen peptide fragment.
Speaker
Kirill V. Berezin
Saratov State University
Russia
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