Оptical clearing of human skin: molecular modeling and in vivo oct study - prospects for multimodal imaging
K. V. Berezin1, E.V. Grabarchuk2, A. M. Lichter2, K. N. Dvoretski3, V. V. Tuchin 1,4; 1Institute of Physics, Saratov State University, Saratov, Russia, 2Astrakhan Tatishchev State University, Russia, 3Saratov State Medical University, Russia, 4Science Medical Center, Saratov State University, Saratov, Russia
Abstract
The results of in vivo immersion optical clearing of human skin under the action of two different optical clearing agents (OCAs), such as an aqueous sucrose solution and a radiographic contrast agent OmnipaqueTM 300, were obtained with the use of OCT method. The rate of reduction of light scattering coefficient, obtained through an averaged A-scan of the OCT image in the region of dermis within the depths from 350 to 700 µm, were determined to evaluate the efficiency of optical clearing (EOC). The correlations between the EOC and the energy of intermolecular interaction of OCAs with a fragment of collagen peptide have been established as a result of molecular modeling by quantum chemistry methods HF/STO3G/ DFT/B3LYP/6 311G(d) of a number of OCAs (glycerol, iohexol, sucrose, ribose, fructose, glucose) with mimetic peptide of collagen (GPH)3.
Speaker
Kirill V. Berezin
Saratov State University
Russia
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