TIME-RESOLVED MULTISPECTRAL MACRO-IMAGING: CLINICAL APPLICATIONS
VLADISLAV I. SHCHESLAVSKIY1,2, DIANA V. YUZHAKOVA1, МARINA V. SHIRMANOVA1, WOLFGANG BECKER2; 1Privolzhskiy Research Medical University, Nizhny Novgorod, Russia; 2Becker&Hickl GmbH, Berlin, Germany.
Abstract
In this talk, we present different optical systems based on time-correlated single photon counting that enable macroscopic time-resolved imaging. The systems allow to map the biological samples not only based on fluorescence lifetime contrast, but also on Raman signals. Moreover, we describe a laser scanning system for macroscopic samples that records fully resolved decay curves in the individual pixels, resolves the images in 16 wavelength channels, and records simultaneously at several laser wavelengths. By using confocal detection, the system delivers images that are virtually free of lateral scattering and out-of-focus haze. Image formats can be up to 256 x 256 pixels, and up to 1024 time channels. We demonstrate the performance of the system both on model experiments with fluorescent micro beads, on tumor model in living mice and finally use it for optical express biopsy of lymphatic nodes to differentiate metastatic and normal tissues.
Speaker
Vladislav Shcheslavskiy
Privolzhskiy Research Medical University
Russian Federation
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