Model of fluorescence and quenching of a protein molecule in a plasmonic complex
A. Yakunin1, S. Zarkov1, Yu. Avetisyan1, G. Akchurin1,2, I. Meerovich3, A. Savitsky3, V. Tuchin1,2,4; 1Institute of Precision Mechanics and Control, FRC “Saratov Research Centre of Russian Academy of Sciences,” Saratov, Russia; 2Saratov State University, Saratov, Russia; 3A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia; 4Tomsk State University, Tomsk, Russia
Abstract
A mathematical model of fluorescence and quenching of a protein molecule in the “TagRFP-nanosphere” plasmonic complex is proposed and described, depending on the material and size of the nanoparticle, and the size of the gap. The point dipole approximation is used to model the radiation source. The model includes a sequential solution to the following two problems. Firstly, the electromagnetic field enhancement in the near-field of the metallic nanosphere is determined, which influences the excitation of the fluorescent molecule. Secondly, from the solution of the electrodynamic problem, radiative and non-radiative losses are estimated to determine the quantum yield of the fluorescent protein molecule-plasmonic nanoparticle complex. The solution of the formulated problems is carried out using the finite element method. Numerical results have been obtained that make it possible to determine the conditions for constructing an effective fluorescent plasmonic complex.
The research was carried out within the state assignment of Ministry of Science and Higher Education of the Russian Federation (theme No. 121022000123-8).
Speaker
Alexander N. Yakunin
Institute of Precision Mechanics and Control, FRC “Saratov Research Centre of Russian Academy of Sciences” Russia
Russia
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