Refractometric analysis of blood serum
L. V. Plotnikova1, O. S. Vezo1, A. D. Garifullin1,2 ,
A. Y. Kuvshinov2 , S.V.Voloshin2,3 , A.M. Polyanichko 1,4.
1St. Petersburg State University
2Russian Research Institute of Hematology and Transfusiology
3Kirov Military Medical Academy
4Institute of Cytology of the Russian Academy of Sciences
Abstract
Refractometric analysis is based on measuring the refractive index of samples in various state. When working with biological fluids, refractometry is a fast and fairly accurate alternative to the biochemical analysis of certain parameters, in particular, the total protein of blood serum. Serum contains dissolved substances in concentrations of 80-100 g/l, most of which are proteins. Serum refractometry assumes that the concentration of inorganic electrolytes and non-protein organic compounds does not change significantly from sample to sample, and differences in refractive index primarily reflect differences in protein concentrations.
The dependence of the refractive index of blood serum and its main protein components on temperature was studied using an Abbemat WR/MW refractometer (Anton Paar, Austria) in the temperature range of 15–45°C. The presence of a temperature dependence of the refractive index of both model systems and blood serum samples was shown. The paper discusses the applicability of the approach for the comparative analysis of blood serum samples from healthy donors and patients with oncohematological diseases.
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Speaker
Людмила
научный сотрудник
Россия
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