Antimicrobial photodynamic exposure using indocyanine green conjugated with various nanoparticles
Elena S. Tuchina 1, Grigoriy K. Savelev 2, Boris N. Khlebtsov 3, Nikolay G. Khlebtsov 3
1 Saratov State University, Saratov, Russia
2 St. Petersburg University, Saint Petersburg, Russia
3 Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia
Abstract
Indocyanine green (ICG) is a water-soluble anionic tricarbocyanine dye capable of absorbing near-infrared radiation and fluorescing. ICG can serve as a photosensitizer for photodynamic therapy of both cancers and microbial lesions, producing both in vitro and in vivo cytotoxic effects when used in combination with light around 800-830 nm.
The targeted delivery of the dye and enhancement of its photodynamic properties can be achieved using various nanomaterials. The most logical choice seems to be gold nanoparticles capable of provoking the effect of plasmon resonance under the action of infrared radiation. In our studies, nanocages conjugated with ICG demonstrated a pronounced antimicrobial effect (elimination of 63% of population of Staphylococcus aureus) after irradiation low-intensity IR light (808 nm).
Additional opportunities are acquired by complexes of nanoparticles and a dye cojugated with such functional molecules as antibodies and their fragments. This allows targeted attack of pathogenic microorganisms without damaging neighboring cells and biological tissues.
But the effect of plasmon resonance is not always beneficial, especially when it comes to mild correction of the number of bacterial representatives of the normal microflora of the human body. In this case, it is possible to use other carriers, for example, silicate nanoparticles. In this case, nanoparticles do not interact with radiation due to their optical properties, and the antimicrobial effect is achieved only due to the dye.
Speaker
Elena S. Tuchina
Saratov State University
Russia
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