The experimental study and modeling of liver optical properties in the 350-1300 nm range

Abstract

Currently, the search for parameters and technologies to assess the functional state of the liver and predict liver failure remains relevant. An important stage in the development of new optical devices is the estimation of optical properties and the modeling of light interaction with biological tissues. To analyze the results, it is also necessary to know the optical properties with different content of blood, water, bile, lipids. The aim of this work was to find and experimentally determine the optical properties of liver tissue and subsequently apply them to modeling.
Liver tissue sections from six healthy Wistar rats and bile samples from nine patients with obstructive jaundice were used for optical measurements. In three rats, liver vessels were washed from blood with Hanks' solution to obtain the basal absorption coefficient. A Shimadzu UV-2600 spectrophotometer with an integrating sphere and a custom-designed sample holder with 1.5 mm diameter holes was used to measure total and collimated transmittance and diffuse reflectance. Absorption and reduced scattering coefficients and an anisotropy factor were calculated using the inverse addition-doubling method. Total tissue absorption coefficients were calculated by adding different contents of oxy- and deoxyhemoglobin, water, lipids, and bile with known optical properties. The values of the anisotropy factor were also calculated for different blood volumes and saturations. The set of obtained properties was used for modeling of diffuse reflectance spectra of liver tissues.
The obtained results are of practical value for further development of diagnostic criteria for assessment of functional state of liver in various pathologies.
The study was supported by the Russian Science Foundation under grant № 21-15-00325.

Speaker

Ksenia Kandurova
Orel State University
Russia

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