Surface-enhanced Raman scattering of blood plasma of patients with malignant and benign neoplasms of the endometrium in comparison with the control group
Dmitry N. Artemyev 1, Lyudmila A. Bratchenko1, Vladimir I. Kukushkin 2, Vladimir M. Zuev 3, 1 – Samara National Research University, Samara, Russia, 2 – Institute of Solid State Physics RAS, Chernogolovka, Russia, 3 – Sechenov University, Moscow, Russia
Abstract
Currently, endometrial cancer is the most common cancer of the genital organs in developed countries. Some types of endometrial hyperplasia may progress to this malignancy. Diagnosis of endometrial cancer and hyperplasia (precancerous conditions) is based on histopathological evaluation of tissue samples and is time-consuming, subjective and subject to re-evaluation. Therefore, there is a need to develop objective assessment methods to diagnose early changes. The study is devoted to the analysis of Raman scattering of blood plasma followed by multivariate analysis (PLS-DA) of spectral data for the diagnosis of endometrial cancer, hyperplasia and normal control group.
Registration and analysis of the results of surface-enhanced Raman scattering was carried out using colloidal silver nanoparticles and a microscopic system. The spectral characteristics of the analyzed colloidal medium were analyzed on an experimental bench consisting of a spectrometric system (EnSpectr R785, Spektr-M, Chernogolovka, Russia) based on a CCD detector and a microscope (ADF U300, ADF, China). The spectra were excited in the near IR range using a laser module with a central wavelength of 785 nm.
As a result, using the PLS-DA method, the accuracy of separation of the polyp Raman spectra relative to the control group and hyperplasia for the calibration and validation sets was more than 80%. It is worth noting that these are preliminary studies that show the possibility of detecting diseases and the potential of the method for diagnosing specific types of tissue diseases in the study of the biofluid (plasma) of this patient.
Speaker
Dmitry N. Artemyev
Samara National Research University
Russia
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