Photodynamic action of blue light using pyridylporphyrin (Zn-TOEt4PyP) on clinical strains of Staphylococcus aureus
Tatiana V. Sharabarina1, Anna A. Zakoyan2, Maria V. Korchenova1, Elena S. Tuchina1, Lusine V. Mkrtchyan2, Grigor V. Gyulkhandanyan2, Valery V. Tuchin1; 1Saratov State University, Saratov, Russia, 2Institute of Biochemistry, NAS of Armenia, Armenia, Yerevan
Abstract
The massive overuse and uncontrolled use of antibiotics is leading to the emergence of new antibiotic-resistant strains of bacteria. Clinical strains of Staphylococcus aureus are the most important causative agents of severe nosocomial infections. In modern medicine, antimicrobial photodynamic action is an alternative way to combat diseases caused by both susceptible and resistant bacteria. In this regard, the main goal of this study was to study at low concentrations as a promising photosensitizer for antimicrobial photodynamic action on staphylococci.
Three clinical strains (lab. numbers 2, 5, 9) of S. aureus were used as the studied microorganisms and were grown at a temperature of 37°C on standart nutrient medium.
An LED with a maximum emission spectrum of λ=405 nm and a power density of 80 mW/cm2 was used as a radiation source. In all experiments, the radiation mode was continuous. Irradiation time varied from 5 to 30 min.
Water-soluble meso-substituted cationic pyridylporphyrin Zn-TOEt4PyP was used as a photosensitizer in concentrations 0.01, 0.03, 0.07 g/ml.
Thus, it was found that the studied porphyrin at low concentration 0.03 demonstrating promising properties as photosensitizers for antimicrobial photodynamic therapy. The reasons and mechanisms of the greater sensitivity of S. aureus strain 2 compared to the other two studied strains require further study.
Speaker
Tatiana V. Sharabarina
Saratov State University
Russia
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