EXPRESS METHOD FOR DETERMINING THE CONCENTRATION OF MICROALGAE AND THE CONTENT OF BASIC PLANT PIGMENTS IN THE CULTURE OF DUNALIELLA SALINA IN VIVO
I. P. Lehman 1, L. A. Dykman 2, V. A. Bogatyrev 1.2
1 Saratov State University, 83 Astrakhanskaya St., Saratov 410012, Russia
2 Institute of Biochemistry and Physiology of Plants and Microorganisms Federal Research Center “Saratov Scientifi c Center of the Russian
Academy of Sciences” (IBPPM RAS), 13 Entuziastov Ave., Saratov 410049, Russia
Abstract
Dunaliella is an important genus of microalgae in biotechnology, one of the advantages of which is the production of high-quality β-carotene. In the process of development, the size and shape of cells change, as well as the content of the main plant pigments - chlorophylls a and b, and carotenoids. For the rapid assessment of the concentration of microalgae and the quantitative pigment composition, an original digital photocolorimetric method has been developed. The method is based on digital image processing of culture flasks or multiwell plates. Images can be taken with a smartphone, or a scanner. The results of in vivo measurements are characterized by low sensitivity to an uncontrolled change in light scattering (turbidity of suspensions). The method is based on the analysis of images in the RGB additive color model and makes it possible to obtain photocolorimetric characteristics similar to spectrophotometric ones, i.e. express concentration in terms of units of optical density.
The main result of the work is to identify the feasibility of the Bouguer-Lambert-Beer law - a linear dependence of the optical density of suspensions of living microalgae on concentration and optical path length. Digital photocolorimetric evaluation can be done offline using time lapse photography or online in real time.
Speaker
Лехман Илья Петрович
Saratov State University
Russian
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