STUDY OF DIFFUSION PROPERTIES OF CHITOSAN-BASED NANOCOMPOSITES WITH AG-IN-S2 QUANTUM DOTS, AND TETRAPHENYLPORPHYRIN MOLECULES
Sergey A. Kabanov, 1
Fayza A. Sewid, 2
Anna O. Orlova, 1
1 ITMO University, Saint-Petersburg, Russia
2 Faculty of Science, Mansoura University, Egypt
Abstract
Chitosan is a widespread substance of natural origin. Chitosan nanoparticles can be used as drug delivery systems. During the study, methods for the synthesis of chitosan-based nanocomposites with Ag-In-S2 quantum dots (AIS), and tetraphenylporphyrin molecules and the spatial distribution of photoluminescent (PL) were analyzed. Potentially, such particles shall be able to get into cells without causing nanotoxicity associated with the small size of the objects. The preparation of a solution of chitosan nanoparticles (NPs) was carried out by the method of ionic gelation for water solutions. Two different types of nanoparticles were prepared; in one of the cases, sodium tripolyphosphate was used to crosslink chitosan molecules. The particle sizes were estimated from their diffusion parameters using fluorescence recovery after photobleaching (FRAP) technique. The experiments were performed with laser scanning confocal microscope Zeiss LSM 710 with 405 nm diode laser. Also results of FRAP investigations were compared to those of dynamic light scattering (DLS) measurements. Comparison of the data of two hydrodynamic measurements demonstrated the successful formation of nanoparticles with a diameter of about 100–300 nm. Comparison of sizes obtained by different methods showed the absence of individual molecules and quantum dots in the samples, which indicates the successful formation of nanocomposites.
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Speaker
Sergey Kabanov
ITMO University, Saint-Petersburg
Russia
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