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Ultrasound enhancement of skin optical clearing ex vivo

Daria K. Tuchina,1-3 Alla B. Bucharskaya,4 Valery V. Tuchin1-3,5
1Research-Educational Institute of Optics and Biophotonics, Saratov State University, Saratov, Russia
2Interdisciplinary Laboratory of Biophotonics, Tomsk State University, Tomsk, Russia
3A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russian Federation
4Saratov State Medical University, Saratov, Russia
5Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control RAS, Saratov, Russia


The study was conducted on the ex vivo skin samples of white Wistar rats. Aqueous 70%-glycerol solution with 10% of DMSO was used as an optical clearing agent (OCA). Collimated transmittance of intact samples immersed in OCA was measured. Then the ultrasound with the frequency 1 MHz in pulsed mode and the power 1.5 W was provided to the sample surface for 1 minute by a Dynatron 125 US system (Dynatronics, USA). After that the kinetics of collimated transmittance of samples immersed in OCA was measured and then processed for estimation of glycerol diffusion coefficients in skin. The faster skin optical clearing (2.5±1.0)×10-6 cm2/s was obtained compared to the same experiment without US application.

This work was supported financially by the RF President Scholarship №СП-5422.2021.4, the Tomsk State University Development Programme (Priority-2030).


Daria K. Tuchina
Saratov State University
Russian Federation


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