Laser-formed electrically conductive nanomaterial for cardiac regeneration
Uliana E. Kurilova,1,2 Mikhail S. Savelyev,1,2 Irina A. Suetina,3 Marina V. Mezentseva,3 Leonid I. Russu,3 Alexander Yu. Gerasimenko,1,2
1 I.M.Sechenov First Moscow State Medical University, Moscow, Russia 2 National Research University of Electronic Technology, 3 National Research Center for Epidemiology and Microbiology Named after the Honorary Academician N.F. Gamaleya, Moscow, Russia
Abstract
In this work biocompatible nanomaterial for the regeneration of damaged cardiac tissues was developed. The main component of the material was the framework of carbon nanotubes in biopolymer matrix. The material was formed layer by layer from liquid dispersion of components using laser radiation at a wavelength of 1064 nm, which has an orienting and binding effect on carbon nanotubes. Laser treatment ensured high strength, porosity and electrical conductivity of the framework. Scanning electron microscopy studies revealed the highly porous structure with bimodal pore distribution important for the ingrowth of various cellular structures. The electrical conductivity of the obtained samples increased with increasing laser processing power, which is associated with the formation of a greater number of bonds between nanotubes in the framework. According to the data of Raman spectroscopy, the defectiveness of nanotubes decreased with increasing power, most of the nanotubes were not damaged by laser radiation. The biocompatibility of samples was evaluated in vitro after incubation with cells for 72 hours. Fluorescence microscopy of cells on the surface of the samples showed that the samples provide cell proliferation, and the morphology of cultured cells does not differ from the morphology of cells on control samples, indicating the absence of toxicity.
This work was financed by the Ministry of Science and Higher Education of the Russian Federation within the framework of state support for the creation and development of World-Class Research Centers "Digital biodesign and personalized healthcare" №075-15-2022-304.
Link to report:
https://disk.yandex.ru/i/M5docY0ediOrLw
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Uliana Kurilova
I.M.Sechenov First Moscow State Medical University
Russia
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