Optical methods for studying the effect of light on the microstructure of peas

Abstract

The paper presents the results of experimental studies on the use of optical methods to assess the effect of light on the microstructure of peas. Pea seeds were planted in the ground depending on the light illumination. The first group (1) was planted in the shady side and the second group (2) was planted in the sun. Plants were monitored once a month using optical research methods throughout the entire life cycle of plants. We studied 3 leaves from each group at different periods of the life cycle. Plants were watered weekly.
All studies were carried out using the fluorescence method. This method was implemented on the basis of a Sharmrock S R-303i spectrometer (Andor Technology Ltd., UK), an ANDOR DV-420A-OE digital cooled camera (Andor Technology Ltd., UK) and a computer. A laser diode with a wavelength of 480 nm served as a fluorescence source. An additional optical control method was microscopic analysis, which was implemented using a laser confocal fluorescence microscopy system assembled on the basis of an Olympus IX71 microscope and an ANDOR laser combiner.
To assess the effect of light on the microstructure of peas, the optical coefficient associated with the life cycle of plants was used.
As a result of the experiments, it was found that the highest values of the optical coefficient were observed after 2 months of research in all the studied groups.
With the help of microscopic analysis, changes in the microstructure of pea leaves associated with a change in the number of chloroplasts in different periods of the plant life cycle were established. The largest number of chloroplasts was found in group 2 after 3 months of research.
Using optical methods, it has been shown that solar illumination prolongs the life cycle of plants.

Speaker

Elena Timchenko
Samara University
Russia

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