Application of methods of luminescence-kinetic spectroscopy and dynamic light scattering in the study of complexes of the composition: glycated and non-glycated human serum albumin
Vyacheslav I. Kochubey, Saratov State University
Alexander B. Pravdin, Saratov State University
Denis A. Bykov, Saratov State Technical University
Andrey G. Melnikov, Saratov State Technical University
Gennady V. Melnikov, Saratov State Technical University
Abstract
The study of the luminescence decay kinetics (fast and delayed fluorescence, as well as phosphorescence) of an eosin probe bound by HSA, gHSA globules and HSA-gHSA complexes suggested the existence of HSA-gHSA complexes in solutions of phosphate buffer pH 7.4 in the absence of free glucose.
Our studies using dynamic light scattering methods experimentally confirmed our assumption about the formation of HSA-gHSA complexes.
Speaker
Melnikov Andrey Gennad'evich
Saratov State Technical University
Russia
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