Raman scattering microspectrocopy of malignant skin neoplasms
Alina Timurzieva,1,2 Elena Rimskaya,1 Svetlana Shelygina,1 Irina Saraeva,1 Elena Perevedentseva,1 Nikolay Melnik,1 Konstantin Kudrin,1,3 Dmitry Reshetov,4 Alexey Gorevoy, 1 Sergey Kudryashov,1 1 Lebedev Physical Institute, Moscow, Russia 2 N.A. Semashko National Research Institute of Public Health, Moscow, Russia 3 I.M. Sechenov First Moscow State Medical University, Moscow, Russia 4 A.I. Evdokimov Moscow State University of Medicine and Dentistry, Moscow, Russia
Abstract
Modern healthcare needs the development of methods for the early detection of skin tumors in order to improve the quality of the organization of oncological care. Therefore, a promising way to solve this problem is the use of Raman scattering microspectroscopy with excitation in the visible range (532 nm).
The paper presents an experimental substantiation of the use of the technology of confocal Raman scattering microspectroscopy at 532 nm wavelength in vitro as the first stage in the development of a method for early non-invasive express diagnostics of tumor diseases, in this case, basal cell carcinoma and squamous cell carcinoma of the head and neck. We also analyzed Raman spectra without noise and without fluorescence of skin tumors in vitro, in normal and pathological conditions.It is shown that Raman scattering microspectroscopy with the analysis of Raman peaks has high sensitivity and specificity (>80%). Thus, this method can be effectively used as an objective non-invasive method for diagnosing skin tumors to identify the characteristic pathomorphological signs of tumors for their verification.
Speaker
Alina Timurzieva
Lebedev Physical Institute
Russia
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