Multispectral Raman and photoluminescence microanalysis of malignant skin neoplasms
Elena Rimskaya,1 Svetlana Shelygina,1 Irina Saraeva,1 Elena Perevedentseva,1 Alina Timurzieva,1,2 Konstantin Kudrin,1,3 Sergey Kudryashov,1 1 Lebedev Physical Institute, Moscow, Russia 2 N.A. Semashko National Research Institute of Public Health, Moscow, Russia 3 I.M. Sechenov First Moscow State Medical University, Moscow, Russia
Abstract
Confocal scanning Raman and photoluminescence microspectroscopy is a structure-sensitive optical method that allows non-invasive measuring chemical composition of biomarkers in the skin tissues. We used it to perform in vitro diagnostics of different skin tumors at several excitation wavelengths (532, 785 and 1064 nm). The laser wavelength significantly affects the relationship between the intensities of Raman skin biomarkers bands of various tissue biomolecules. Distinct spectral differences were noticed for Raman spectra of basal cell carcinoma and squamous cell carcinoma compared to healthy skin. The results of the study of Raman/PL spectra at different excitation wavelengths indicated that the spectral criteria, calculated as the ratio of the selected Raman peak intensities, provide useful information assisting in differentiation of healthy skin from BCC and SCC with sensitivity and specificity higher than 95%, which has the greatest clinical importance in differential diagnostics of skin tumors. The multiwavelength optical methods based on joint analysis of the data obtained at three (or more) wavelengths of laser excitation can be helpful for early diagnostics of malignant skin neoplasms.
Speaker
Elena Rimskaya
Lebedev Physical Institute
Russia
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