Photoinduced NO generation and controlled release from BODIPY-doped polymer films

Abstract

Nitric oxide (NO) is a signaling gaseous molecule involved in a wide range of physiological and pathological processes. Photodynamically activated NO donor species, particularly those that may also generate ROS, are very attractive. However, there are potential problems with the use of such NO photodonors in medicine: high concentration of such compound can be reached in organic solvents only, due to aggregation in aqueous solution, and the photodonor itself can be toxic or even cancerogenic since it contains N-Nitroso bond.
In this study, we focus on the investigation of BODIPY-based NO photodonors incorporated into the polymer matrix. PDMS (Sylgard 184) (10:1 base:curing agent ratio) was mixed with dye concentration 75uM (50uL buffer solution in methanol with concentration 1.5mM on 1ml of PDMS). The photolysis was done with high-power LEDs (500 nm central wavelength) or with 488 nm, 50 mW continuous laser (CrystaLaser DL488-050). The intensity of sensor response depended on the laser power, which was from 1 to 5% of maximum, corresponding to 0.5 – 2.5 mW. The light-induced NO-release properties was studied with three different methods: electrochemical gas-phase and liquid-phase sensors and Griess assay. We showed that the photodonors in such environment are capable of NO photorelease with better efficiency compared to organic solvents. We observed the prolonged release of photogenerated NO from polymeric matrix due to passive diffusion, which also could be beneficial for NO-based therapy.
Acknowledgements. The study was supported by the Russian Science Foundation (grant #23-75-10049)

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Natalia Virts
Novosibirsk State University
Russian Federation

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