COMPLEX APPROACH TO IN VITRO AND IN VIVO MONITORING OF THE DEGRADATION OF IMPLANTS BASED ON ESTER COPOLYMERS USING MAGNETIC RESONANCE AND FLUORESCENCE IMAGING
Astemir R. Likhov,1, Natalia I. Каzachkina ,1, Dmitry D. Demin,3, Veronika N. Volodina,1 ,Victoria V. Deeva,1, Ilya D.Solovyev,1, Nadezhda N. Marynich,1, Irina G. Meerovich,1, Daria K. Tuchina,1,2, Valery V. Tuchin,1,2 and Victoria V. Zherdeva1.
1-RC of Biotechnology of the RAS, Moscow, Russia;
2-Saratov State University, Saratov, Russia;
3-MIREA — Russian Technological University, Moscow, Russia.
Abstract
Copolymer-based materials are widely used in medicine and biotechnology to produce various medical products, such as implants, prostheses, drug delivery systems, sensors and even triboelectric nanogenerators. An important aspect when using such materials is their biodegradation and bio resorption taking into account that results of biodegradation may show high variation.
The aim of our work was to understand the preliminary mechanisms of degradation demonstrated by the ester copolymers in vitro and in vivo and to show the possibility of visualization these copolymers in vivo using bimodal approach as MRI and fluorescence imaging.
Copolymers citric acid, 1,3-propandiol and 1,5-pentadiol were synthetized and marked with indocyanine green and paramagnetic resonance agent like Gd-DOTA or Gd-citrate complex.The inclusion of fluorescent and paramagnetic agent into the copolymer was confirmed. The polymers showed no cytotoxic properties while maintaining stable physiological pH values.
Polymers enzyme-mediated hydrolysis was modeled in vitro and the correlation between fluorescence and MRI signal change have been demonstrated. The process of degradation was differed in a buffer and in a serum. Enzyme-mediated hydrolysis in a serum was about 3 times slower than enzyme-mediated hydrolysis in a buffer.
The implant compatibility in mice BALB/c has been demonstrated. It has been shown that ICG fluorescence as well as MRI was detected up to 28 days.
Thus, we demonstrated possibility to mark and visualize ester copolymers by fluorescence imaging and MRI in vitro and in vivo.
This research was funding by Ministry of Science and Higher Education of the Russian Federation grant № 13.2251.21.0009 from 29.09.2021 (Agreement № 075-15-2021-942).
Speaker
Zherdeva Victoria
RC of Biotechnology of the RAS
Russia
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