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The Near-lnfrared-II fluorescence Imaging Probes for Surgical Navigation

1 Mengchao Hepatobiliary Hospital of Fujian Medical University, Fujian Fuzhou, P. R. China


Fluorescence surgical navigation technology depends on fluorescence imaging system and fluorescence probe to display focus information more accurately during operation. Currently, the fluorescent surgical navigation probes approved for clinical use include sodium fluorescein, 5-aminolevulinic acid (5-ALA), methylene blue (MB) and indocyanine green (ICG). However, the excitation and emission wavelengths of sodium fluorescein, 5-ALA and MB locate in the 400-700nm region. As a result, the penetration depth is very limited, only tissue surface imaging can be performed, and the signal-to-noise ratio is also very low. Therefore, the application of these probes in surgical navigation has been gradually eliminated in clinic. In order to obtain deeper tissue penetration depth and carry out more accurate fluorescence imaging and surgical navigation, it is necessary to use near-infrared fluorescence probes with longer excitation and emission wavelengths. Indocyanine green (ICG) is currently the most widely used and thoroughly studied near-infrared fluorescence molecular probe in fluorescence surgical navigation. ICG, which was first synthesized by Kodak Laboratory in Japan in 1955, can be excited by light at the wavelength of 750 ~ 810nm and emit near-infrared light with the maximum wavelength of 830nm. The near infrared fluorescence surgical navigation technique based on ICG has been widely used in the field of tumor surgery, and many clinical studies have confirmed its clinical value in the aspects of surgical thoroughness, surgical convenience, recurrence-free survival, and total survival. However, NIR-I fluorescence imaging still has a series of inherent bottlenecks, such as low penetration depth, high background signal, poor signal-to-noise ratio and so on. In recent years, with the deepening of NIR-II fluorescence imaging research and the rapid development of nanotechnology, a series of organic small molecules, organic nano-probes and inorganic nano-probes have been developed and applied as NIR-II fluorescent molecular probes in the fields of fluorescence imaging and surgical navigation. We will present the recent developed NIR-II fluorescence imaging probes for surgical navigation in our group.


Xiaolong Liu
Mengchao Hepatobiliary Hospital of Fujian Medical University


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