Tissue Optical Clearing for Whole-organ Imaging
Acquiring the three-dimensional (3D) structure of biological tissues is essential for research in life sciences. Modern optical imaging techniques and fluorescent labeling technologies have provided vital tools for obtaining high-resolution information on the 3D structures of biological tissues. However, the turbid nature of biological tissues limits the depth of light penetration, leading to restricted applications for large tissues or whole organs. Tissue optical clearing technology takes a different approach by making the tissues transparent using various physical and chemical strategies to reduce the attenuation of light in tissues, and providing a new approach for the 3D imaging of whole tissue organs. Here, we will introduce our progress in the research of tissue optical clearing methods and applications, covering the fluorescence labeling, tissue clearing, and imaging of large tissues/whole organs.
Huazhong University of Science and Technology