EFFECTS OF NEAR-INFRARED PHOTOBIOMODULATION AT 830 NM IN A DIABETIC WOUNDED CELL MODEL
Hadebe L and Houreld N; University of Johannesburg, Laser Research Centre, South Africa
Abstract
Diabetic wounds present a significant healthcare burden due to their failure to progress through the usual phases of healing within the expected time, leading to decreased cellular migration, skin ulceration, and impaired wound healing. Complications arising from these wounds, such as lower extremity amputation, can decrease patient quality of life and result in premature death. Photobiomodulation (PBM), previously known as low-level light therapy (LLLT) or cold laser therapy, is a non-invasive medical treatment that uses low-intensity light sources to stimulate cellular activity and aid healing in various tissues. This therapy has been researched for its ability to aid wound healing, decrease pain and inflammation, and increase tissue repair. However, the mechanisms that aid the induction of cell migration and survival using an 830 nm laser are poorly understood. Therefore, the present study was designed to evaluate the therapeutic efficacy of near-infrared (NIR) PBM at 830 nm in wounded and unwounded human skin fibroblast (WS1) cell models. Models were exposed to an 830 nm diode laser at a fluence of 5 J/cm2, unirradiated models (0 J/cm2) were used as controls. Post-PBM, the Trypan blue exclusion assay and CellTiter-Glo® 3D Cell Viability Assay (measures adenosine triphosphate (ATP) luminescence) were used to determine the population of viable cells at 24 and 48 h. Morphological changes and migration rate in wounded models was also evaluated at 0 h and 24 h. PBM significantly increased cellular migration and enhanced cell viability, thus improving wound healing in diabetic conditions in vitro at 830 nm.
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Lindokuhle Hadebe
University of Johannesburg
South Africa
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