PHOTOBIOMODULATION PROMOTES WOUND HEALING IN A DIABETIC CELLULAR MODEL

Abstract

Wound healing is a critical process that activates tissue repair after injury. In diabetes mellitus however, this process is perturbed and leads to the development of non-healing diabetic ulcers. Current treatments which include glycemic control wound debridement are moderately effective as there is a challenge of recurrence. Therefore, improved therapeutics strategies are required for the treatment of diabetic ulcers. Photobiomodulation (PBM) at the wavelength range of 520 and 830 nm has been shown to improve the healing of cutaneous wounds by promoting cell proliferation and wound closure. The aim of this study was to determine if PBM at the wavelength of 660 nm and a fluence of 5 J/cm2 can induce wound healing in a diabetic (D) cellular model. Human dermal fibroblasts were continuously cultured in high-glucose environment to induce the diabetic state. Prior to irradiation, a central scratch was created on the cell culture plates to create the “wound”. Cell migration and cell viability analyses were performed 24 and 48 h post-irradiation. The results showed that cell migration was increased in irradiated diabetic wounded (DW) cells compared to the non-irradiated cells. The trypan blue and MTT data showed no statistical difference in cell viability between irradiated and non-irradiated cells at 24 and 48 h post-irradiation. The preliminary findings of this study indicate that PBM does not cause significant cell death and promotes “wound” closure in diabetic wounded cells.

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Speaker

Dimakatso Gumede
University of Johannesburg
South Africa

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