Time-resolved polarized fluorescence of NADH and FAD in solutions: picosecond relaxation dynamics
Oleg S. Vasyutinskii
Ioffe Institute Russian Academy of Sciences
St.Petersburg, Russia
Abstract
Nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) are essential biological coenzymes involved in regulation of living cell metabolism that are widely used nowadays as fluorescent biomarkers for monitoring the respiratory chain activity. The lecture presents the results of experimental and theoretical studies of anisotropic relaxation and energy transfer in excited states of NADH and FAD in solutions under excitation with pico- and femtosecond laser pulses. Time-resolved transient polarization-modulation and fluorescence anisotropy signals were recorded and analyzed by means of the methods developed by the authors. The studies have brought a new insight on several important aspects of excited states quenching and anisotropic relaxation. The results obtained and methods developed can be used for investigation of redox reactions in living cells.
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Oleg Vasyutinskii
Ioffe Institute, St.Petersburg
Russia
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