The Effect of Clustering of Gold Plasmonic Nanoparticle–protein complexes as a Factor in Increasing the Fluorescence Intensity
A.N. Yakunin,1 S.V. Zarkov,1 Yu.A. Avetisyan,1 I.G. Meerovich,2 V.V. Tuchin,1,2,3,4 A.P. Savitsky,2; 1 Institute of Precision Mechanics and Control, FRC “Saratov Scientific Centre of the Russian Academy of Sciences”, Saratov, Russia; 2Bach Institute of Biochemistry, FRC “Fundamentals of Biotechnology of the Russian Academy of Sciences”, Moscow, Russia; 3Saratov State University, Saratov, Russia; 4Tomsk State University, Tomsk, Russia
Abstract
The effect of clustering of gold plasmonic nanoparticle–protein complexes as a factor in increasing the fluorescence intensity has been studied. It has been shown that a change in the structure of the near field in the vicinity of complexes in clusters of various types leads to an increase in the integral excitation power of the fluorescent TagRFP protein. In our works, we theoretically studied the interaction of laser radiation with complexes of gold plasmon resonance nanospheres (GNS) with the TagRFP protein without taking into account the mutual near-field interaction of such complexes. The aim of this work is to evaluate the influence of this interaction on such characteristics as field amplification and absorption of laser radiation by clusters of GNS-TagRFP complexes. Naturally, these characteristics actually determine the intensity of the object's fluorescent response to photoexcitation. Therefore, for a comparative evaluation of the efficiency of GNS-TagRFP plasmonic protein complexes in clusters, the results of calculating the field distribution carried out for the wavelength λ of the protein photoexcitation maximum, namely λ=558 nm, are important. It is found that in each of the considered clusters, the field localization zones are redistributed: in the region of the closest approach of the complexes, they are characterized by an increase in the field maximum compared to the case of a solitary complex. Depending on the type and number of complexes in a cluster, the field maximum can increase up to 2 times. Accordingly, the non-uniformity of the specific absorption power also increases, which should be taken into account in the possible applications of the GNS-TagRFP complexes.
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Alexander N. Yakunin
Institute of Precision Mechanics and Control, FRC “Saratov Scientific Centre of the Russian Academy of Sciences”, Saratov
Russia
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