Biocompatible optical clearing of murine melanoma ex vivo and in vivo
Sergey M. Zaytsev, 1 Natalya A. Shushunova, 1 Isabella A. Serebryakova, 1,2 Yu. I. Surkov, 1,2 Elina A. Genina, 1,2 1 Saratov State University, Saratov, Russia 2 Tomsk State University, Tomsk, Russia
Abstract
The study is devoted to application of skin optical clearing technique for murine melanoma improved detection in vivo and ex vivo by the means of Optical Coherence Tomography, Diffuse Reflectance spectroscopy and Dermoscopy. In this work we used 8 different combinations of optical clearing agents and chemical permeation enhancers for increasing the optical probing depth in 2 different conditions (in vivo and ex vivo). All mixtures used met the FDA maximum concentration requirements for use on the skin. Eight albino laboratory mice with model melanoma were used in this study. Dermabrasion was applied to the lesional skin site as physical permeation enhancer before the mixture application. Mechanical massage was used to enhance the penetration of chemicals. Optical Coherence Tomography (OCT), Diffuse Reflectance Spectroscopy and Dermoscopy were used to measure the changes in vivo and ex vivo in skin probing depth.
Speaker
Sergey Zaytsev
Saratov State University
Russia
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