The study of bile composition by Raman spectroscopy in patients with obstructive jaundice
Vadim N.Prizemin,1 Nadezhda V. Golubova,1 Dmitry S. Sumin,2 Ksenia Y. Kandurova,1 Andrian V. Mamoshin,1,2 Elena V. Potapova,1 1 Orel State University named after I.S. Turgenev, Orel, Russia 2 Orel Regional Clinical Hospital, Orel, Russia
Abstract
Timely determination and prediction of the development of liver dysfunction is important for improving the results of treatment of patients with biliary tract obstruction. Currently, various nonspecific scales are used to assess the functional state of the liver in obstructive jaundice, the disadvantages of which are the lack of standards and the non-statistical nature of prognosis.
The synthesis of bile acids, the release of bilirubin and its conjugation with glucuronic acid are specific functions of the liver. The study of these substances in hepatic bile in order to identify the functional state of the liver in obstructive jaundice is of considerable interest for predicting the course of the disease. Bile obtained during antegrade decompression of the biliary tract can serve as a basis for rapid and highly sensitive diagnosis.
The results showed that the inhibition of the excretory function of hepatocytes in obstructive jaundice affects the level of bilirubin in bile. A significant decrease in the amplitude of the peaks of bilirubin in the characteristic bands of Raman scattering (1615 cm-1, 1264 cm-1) was observed. The data obtained correlated with the level of bilirubin fractions and liver enzymes in the blood, the duration of obstructive jaundice and the etiology of biliary tract obstruction. The proposed approach may be promising in assessing the functional state of the liver and predicting the course of the disease.
The study was supported by the Russian Science Foundation under the project №21-15-00325.
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Vadim Prizemin
Orel State University named after I.S. Turgenev
Russia
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