Possibilities of hyperspectral imaging to assess the intestinal ischemia degree

Abstract

Today, the development of new methods for assessing tissue viability during surgical intervention is a promising area of development in modern biomedical engineering. This study investigates the application of hyperspectral imaging technology for the analysis of intestinal tissue ischemia in the laboratory rat in the simulation of local ischemia.
The study objects were three Wistar line healthy laboratory rats. The developed optical imaging method based on the registration of diffuse light reflection from tissues using a hyperspectral approach. A developed halogen source used as a broadband source, and a hyperspectral camera Specim (Spectral Imaging Ltd., Finland) - as a detector.
In accordance with the protocol of the study, a midline abdominal laparotomy performed in the laboratory rat, followed by isolation of the small intestine. Then a ligature applied to the intestinal arcade vessels and the intestine immersed back into the abdominal cavity. Measurements of the occluded intestine taken 1, 5, and 12 hours after ligation, respectively. Hyperspectral image arrays of the small intestine obtained. To normalize the measured data, we recorded spectra from the diffuse reflectance etalon.
Based on the different light absorption by oxygen unbound / bound forms of hemoglobin, the tissue saturation parameter calculated using a two-wave approach for the near-infrared region. The difference in the calculated saturation values for the occluded regions for each stage of measurement indicates the possibility of using hyperspectral imaging to assess the degree of intestinal ischemia.
The study was supported by the Russian Science Foundation under the project № 21-15-00325.


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Speaker

Ilya Goryunov
Orel State University named after I.S. Turgenev
Russia

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