Development of methods for the determination of allergens using microstructural waveguides
Daria V. Shumarina 1, Julia S. Skibina 2, Yuliya D. Gudova 1,2, Pavel A. Lepilin 1,2, Svetlana S. Konnova 2 1 Saratov State University, Saratov, Russia, 2 LLC SPE “Nanostructured Glass Technology”, Saratov, Russia
Abstract
In recent years, optical waveguide in the form of biosensors has become increasingly popular. The convenience of this method lies in the fact that for an experiment using biosensors based on a microstructural waveguide, ≈ 30 µl of the liquid under study is required, which is several times less than with the conventional ELISE method. In addition, waveguide-optic biosensors are very sensitive, which makes it possible to determine a small difference in the concentrations of the studied samples.
Optical waveguide can be used to measure the absorption and transmission spectrum of liquids with different densities and refractive indices.
In this work, an experiment is carried out to detect IgE antibodies in human blood using the curve of calibration samples of various IgE concentrations using microstructural waveguide.
Class E immunoglobulins are antibodies that take part in the development of an allergic reaction. An increase in the level of immunoglobulin is observed in asthma, as well as in hay fever and other allergic manifestations. Therefore, the diagnosis of allergies in humans is important to prevent allergic complications.
Speaker
Daria V. Shumarina
Saratov State University
Russia
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