Visualisation of vascular changes and assessment of blood flow variations by direct optical generation of singlet oxygen
Lyubov V. Eratova1, Denis I. Myalitsin2, Mikhail V. Volkov2, Viktor V. Dremin1, Irina N. Novikova1, 1 Orel State University, Orel, Russia, 2 University ITMO, Saint-Petersburg, Russia
Abstract
The study of the effect of reactive oxygen species (ROS) on the human body is a topical issue in the scientific community. One of the ROS called singlet oxygen (SO) is of particular interest due to its high reactivity. Currently, ROS is known to cause changes in vascular tone, but studies do not allow one to attribute the main role of SO in this process. This is due to the toxicity of photosensitizers used during therapy and the possible generation of ROS other than SO. Direct optical SO generation will allow the influence of these factors to be excluded, thus contributing to the development of fundamental knowledge about the importance of SO in vascular regulation and expanding the field of laser medicine application. Thus, the scientific purpose has been to study changes in vasculature parameters under the effect of SO during its direct optical generation.
For the in vivo study of the effect of direct optical SO generation on changes in vascular bed parameters, an approach and experimental installation of direct optical SO generation at a wavelength of 1267 nm and visualization of vascular changes have been proposed. The registration of changes in the vascular bed is carried out by means of the optical subsystem under illumination by incoherent (525 nm) and coherent (660 nm) light sources. The installation and the proposed processing algorithm of the obtained images make it possible to reconstruct the photoplethysmogram (PPG) signal and restore the maps of the vessels and their blood filling from the existing set of speckle images for any time point. Additional filtering of the PPG signal enables to study rapidly changing processes caused by blood flow oscillation mechanisms of different nature.
Studies of the vasculature of Wistar line rats in the femoral-gluteal region confirmed that the proposed approach makes it possible to visualize vascular changes during direct optical SO generation and identify the contribution of myogenic, respiratory and cardiac components of blood flow oscillations to the resulting signal.
The work was supported by the Russian Science Foundation under project No. 21-75-00086.
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Lyubov Eratova
Orel State University
Russia
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