Raman spectroscopy investigations of functional state of unicellular organisms
Elena Perevedentseva1, Elena Muronets2, Artashes Karmenyan3, Chia-Liang Cheng3, Irina Elanskaya2, Nikolai Melnik1
1 P.N. Lebedev Physical Institute Rus. Acad. Sci., Moscow, Russia
2 Biological Dept of Moscow State University, Moscow, Russia
3 Department of Physics, National Dong Hwa University, Hualien, Taiwan
Abstract
Raman spectroscopy investigations of functional state of unicellular organisms
Elena Perevedentseva1, Elena Muronets2, Artashes Karmenyan3, Chia-Liang Cheng3, Irina Elanskaya2, Nikolai Melnik1
1 P.N. Lebedev Physical Institute Rus. Acad. Sci., Moscow, Russia
2 Biological Dept of Moscow State University, Moscow, Russia
3 Department of Physics, National Dong Hwa University, Hualien, Taiwan
The Raman spectra of unicellular organisms contain quite specific fingerprint-like signatures. Currently Raman spectroscopy of microorganisms is developing first as a tool for detection and identifying pathogenic or non-pathogenic (e.g. contaminating) microorganisms. Moreover it is also a tool for fundamental studies including the probing of microorganisms, molecular species and analyzing physiological state/processes and metabolic reactions.
In the presented work we discuss applications of Raman spectroscopy for study of the unicellular organisms. Previously we studied a life cycle of unicellular organisms on the example of an amoeba (Acanthamoeba polyphaga) [1] and observed and analyzed the changes of the spectra during the amoeba cyst activation and excystation. We also discussed using Raman for study of pathogenic bacteria (gram-negative E. coli) response to interaction with ultrafine nanodiamond [2]. In the presented work we are focusing on IR-Raman analysis of chemical composition and variations in light harvesting antenna complex of various cyanobacteria strains (wild type of Synechocystis sp. PCC 6803, CK and PAL mutants deficient in phycobiliproteins, mutants deficient on flavodiiron protein Flv3 and succinate dehydrogenase). Using IR Raman spectroscopy for analyzing the molecules and molecular complexes which participate in the cyanobacteria photosynthesis is presented.
Raman spectroscopy is considered in general like life-compatible; however the influence of excitation laser on the microorganism can be a limiting factor. In the presented work conditions and principles for safe and non-damaging measurements and using the unicellular models for these studies are discussed.
[1] Lin et al, J Biomed Optics 20(5), 51042 (2015)
[2] Chatterjee et al, J. Biomed Optics, 20(5), 051014 (2014)
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Elena Perevedentseva
P.N. Lebedev Physical Institute of Rus Acad Sci
Russia
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