Antibacterial effect of nanoparticles on foodborne bacterial biofilms by nanosecond laser-induced forward transfer
Alena A. Nastulyavichus,1 Sergey I. Kudryashov,1 Etery R. Tolordava, 1,2 Yulia K. Yushina, 3 Anastasia A. Semenova, 3 Andrey A. Ionin, 1 1 Lebedev Physical Institute,Moscow, Russia 2 N. F. Gamaleya Federal Research Centre of Epidemiology and Microbiology,Moscow, Russia 3 Federal State Budgetary Scientific Institution "Federal Scientific Center for Food Systems named after V.M. Gorbatov "Russian Academy of Sciences, Moscow, Russia
Abstract
The most bacteria lives in the form of the biofilm and costs humanity lots of infections and diseases [1]. Biofilms are ubiquitous in nature. The formation of biofilms was observed in most bacteria in natural, clinical and industrial conditions. For the food industry it is vital to have bacteria under control, and it’s important to not only maintain existing ways but also research for new ones, since well-known pathogens may adapt, and the emerging pathogens may be already resistant to what’s currently in use [2].
In this work, laser-induced forward transfer of antibacterial silver and copper nanoparticles onto biofilms of Gram-positive and Gram-negative food bacteria was studied. For this Yb3+ doped fiber laser HTF Mark, Bulat (central wavelength λ = 1064 nm, pulsewidth FWHM τ = 120 ns), equipped with a galvanometric scanner and an f-theta objective (focal length F = 160 mm) was used. For research, the standard microbiological method for determining CFU (colony forming unit) was used. By the method of energy dispersive X-ray spectroscopy, it was shown that nanoparticles did not penetrate into the entire biofilm and thus acted on it indirectly from outside. Experimental studies have shown that the use of the method of application laser transfer of silver and copper nanoparticles is an effective method in the fight against biofilms formed on a solid substrate.
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Nastulyavichus Alena
P.N. Lebedev Physical institute of the Russian Academy of Sciences
Russia
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