Inactivation of pathogenic bacteria by mid-infrared laser pulses
Svetlana N. Shelygina,1, Yuri M. Klimachev,1, Eteri R. Tolordava,1,2, Sergey I. Kudryashov,1, Yulia K. Yushina4
1 Lebedev Physical Institute, Moscow, Russia;
2 National Research Center for Epidemiology and Microbiology. N.F. Gamalei, Moscow, Russia;
3 V.M. Gorbatov Federal Scientific Center for Food Systems, Russian Academy of Sciences, Moscow, Russia
Abstract
This work is continuation of investigation of using of mid-IR femtosecond laser irradiation to inactivation of pathogenic microorganisms. Our previous studies have shown the high efficiency of direct laser inactivation of food pathogenic bacteria in the mid-IR range by femtosecond laser pulses with a wavelength of 3 and 6 μm. In this work pathogenic bacteria, that placed on fluorite substrates as a submonolayer and covered by polyethylene film, were irradiated by mid-IR femtosecond laser irradiation with selective wavelength, corresponds to the high absorption efficiency of the bacterial cell and the minimum absorption of the polyethylene film. These wavelengths correspond to the stretching C-H vibrations (3 mkm), or stretching C=O, C-N vibrations (6 mkm) of amide groups of bacteria. This experiment simulates mid-IR laser disinfection of food in polyethylene packing.
The research was supported by the Ministry of Science and Higher Education of the Russian Federation (Project No. 075-15-2020-775).
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Svetlana Shelygina
Lebedev Physical Institute RAS, Moscow, Russia
Russia
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