OCT detection of cervical carcinoma response to therapy
Maria M. Loginova,1,2 Arseniy L. Potapov,1 Ekaterina V. Gubarkova,1,2, Ksenia A. Achkasova,1 Alexandra Yu. Bogomolova,1,2, Stefka G. Radenska-Lopovok,3 Nadezhda V. Kanishcheva,4 Sergey V. Gamayunov,4 Vladimir Y. Zaitsev,5 Natalia D. Gladkova,1 Marina A. Sirotkina,1,2 1 Privolzhsky Research Medical University, Nizhny Novgorod, Russia 2 Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia 3 I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia 4 Nizhny Novgorod Regional Oncologic Hospital, Nizhny Novgorod, Russia 5 Institute of Applied Physics of the Russian Academy of Sciences, Nizhny Novgorod, Russia
Abstract
Cervical cancer is still a common cause of mortality worldwide. Currently, radical radiotherapy of cervical cancer is represented by of external-beam radiotherapy, brachytherapy and chemotherapy with cisplatin. However, even with concurrent chemoradiotherapy and brachytherapy, patients continue to have a poor prognosis. An important task is the development of new methods to control the tumor response to therapy. Optical coherence tomography (OCT) allows to determine the optical properties of tissue, which can be useful in determining markers of early cervical cancer response to therapy.
Therefore, the aim of the research was to reveal the statistically significant changes of optical properties of cervical cancer under chemoradiotherapy.
The object of the research was biopsy samples of cervix squamous cell carcinomas taken from patients before treatment, after the stage of external-beam radiotherapy and after the stage of brachytherapy. The evaluation of the optical properties of cervical cancer tissue was performed using the multimodal optical coherence tomograph developed by the Institute of Applied Physics of the Russian Academy of Sciences (Nizhny Novgorod, Russia). Quantitative analysis of OCT images was carried out by calculating the signal attenuation coefficient in co-polarization and cross-polarization channels. The resulting color-coded OCT maps were compared with histological images.
Before radiation therapy in biopsy specimens, histological analysis showed foci of tumor cells, limited by connective tissue. After the stage of external-beam radiotherapy, sclerosis and fibrosis are observed. After the stage of brachytherapy are detected by coagulative necrosis. Statistically significant differences were revealed (p <0.02) in the optical properties of cervical cancer tissue before treatment, after the stage of external-beam radiotherapy and after the stage of brachytherapy.
Therefore, OCT looks promising method for detecting statistically significant changes of optical properties of cervical cancer during chemoradiotherapy.
The study was supported by Center of Excellence «Center of Photonics» funded by The Ministry of Science and Higher Education of the Russian Federation, contract No 075-15-2020-927.
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Speaker
Maria Loginova
Privolzhsky Research Medical University, Nizhny Novgorod, Russia
Russia
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