Photoconvertible fluorescent capsules as a new approach to the study of cell migration processes, their behavior and functions in fundamental biomedicine
Olga A. Sindeeva, 1, Polina A. Demina, 2, Anastasia Yu. Sapach, 1,3, Zhanna V. Kozyreva, 1,3, Ekaterina S. Prikhozhdenko, 2, Anna M. Abramova, 1, Irina Yu. Goryacheva, 1, Gleb B. Sukhorukov 1,4 1 Skolkovo Institute of Science and Technology, Skolkovo Innovation Center, 143025, Moscow, Russia 2 Sechenov First State Medical University, 8/2 Trubetskaya Str., 119991 Moscow, Russia 3 Saratov State University, Saratov 410012, Russia 4 Queen Mary University of London, London E1 4NS, U.K.
Abstract
In modern biology and biomedicine, there is considerable interest in optical labeling of individual cells in large cell populations. It comes from applied problems: selection of phenotypically competent and highly reliable leader cells in populations of cancer cells, selection of phenotypically identical cells across the heterogeneous cell lines in order to create purified cell lines, selection and obtaining clones of the most viable cells for tissue regeneration and treatment of autoimmune diseases. The fundamental interests in science: to study cell behavior, movement of cells in natural and bioengineered 3D matrices, investigation of mechanisms and principles of interaction between individual cells and within their groups in tissues. A fundamentally new technology, based on the creation of a photoconvertible biocompatible bright label incorporated into cells opening up new ways of working with zero and first passages of primary cell cultures, without the need for their genetic modification and long-term cultivation. It is especially important in problems related to stem and neuronal cells.
This work was supported by the Russian Science Foundation (project no. 22-15-00292).
Speaker
Olga A. Sindeeva
Skolkovo Institute of Science and Technology
Russia
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