Using of T-lymphocytes as a courier for composite polymer/MNPs nanocapsules

Abstract

The development of methods for targeted delivery of cells to target tissues would promote the evolution of localized cell therapy, by which functionalized cells can repair damaged tissue or for anti-cancer therapy treatment strategy. Unfortunately, cell targeting by guiding molecules at this stage of science and technology development does not show such outstanding results, which makes it necessary to look for other methods of cell guidance.In the present study, we implement a technique for functionalization of Jurkat cells (an immortalized T-lymphocyte cell line) using biocompatible submicron capsules with magnetite nanoparticles incorporated into their structure to make the cells magnetically sensitive.The research on functionalization of model T-lymphocytes was performed with magnetic capsules TA/BSA-Cy5 for 500 nm and 300 nm, DEX/PARG-Cy5 for 150 nm capsules. As a result, it was possible to magnetically manipulate these cells in the phantom of microcirculatory networks using magnetic tweezers. The 300 and 500 nm capsules have been shown both to attach externally to the membrane of model T-lymphocytes, while the 150 nm particles penetrate directly into the T-cells.The approach presented in this work opens perspectives for tissue regenerative medicine and anti-cancer studies.

Speaker

Maxim A. Kurochkin
Skolkovo institute of science and technology
Russia

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