The effect of photodynamic therapy on wound healing in diabetes using Raman Spectroscopy in vivo
H. Zuhayri, A.A. Samarinova, D.A. Lopez Guardado, N.A Krivova, Yu.V Kistenev*
National Research Tomsk State University, Lenin Ave. 36, 634050, Tomsk, Russia
Abstract
Photodynamic therapy (PDT) is a modern and non-invasive form of therapy, used in many fields of medicine. PDT proven to be effective in wound healing, and researches tend to study the challenges of the wound healing process, for example diabetes. Raman spectroscopy is a noninvasive optical method that has promise for characterization of the physical properties of wounds and to accurately identify different phases of wound healing at different time-points.
The purpose of this work is to study the effect of photodynamic therapy on the wound healing process in diabetes using two different photosensitizers 5-aminolevulinic acid and methylene blue, and two laser doses 1J/cm2 and 4 J/cm2 by Raman spectroscopy. Raman spectra were collected from full thickness dermal wounds in mice at 4 time-points (1, 3, 7, and 14 days) corresponding to different phases of wound healing.
The research was carried out with the support of a grant under the Decree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021).
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Speaker
Hala Zuhayri
Tomsk State University
Russia
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