Effect of annealing on the Cytotoxicity of Upconversion Nanoparticles in Different Cell Lines
Roman A. Verkhovskii,1 Roman A. Anisimov,1 Jamal R. Kilichev,2 Farid K. Kurbanaliev,2 Roman A. Suldinsky,2 Maria V. Lomova,1 Nikita A. Navolokin,2,3,4 Vyacheslav I. Kochubey,5,6 Irina Yu. Yanina5,6 1Education and Research Institution of Nanostructures and Biosystems, Saratov State University (National Research), Russian Federation
2Department of Pathological Anatomy, Saratov State Medical University, Saratov, Russian Federation 3 Research-Scientific Institute of Fundamental and Clinic Uronephrology, Saratov State Medical University, Saratov, Russian Federation 4 Pathological Department, State Healthcare Institution "Saratov City Clinical Hospital No. 1 named after Yu.Ya. Gordeev" st. them. Kholzunova A.I., Saratov, Russian Federation 5 Department of Optics and Biophotonics, Saratov State University (National Research), Saratov, Russian Federation 6 Laboratory of laser molecular imaging and machine learning, Tomsk State University (National Research), Russian Federation
Abstract
Carriers based on upconversion nanoparticles (UCNPs) and cyanine dye are suitable for theranostic application in oncology, although care must be taken for selection of the surface coating material, UCNP surface charge, size, and dosage of the material. Investigation of influence of annealing temperature of particles on the upconversion luminescence properties and cytotoxic effect are relevant. The present work demonstrates the assessment of cytotoxicity UCNPs unannealed and annealed at 550 oC on different normal and cancer murine cell lines in vitro. The cell viability is scored for cytotoxic effects of UCNPs at dark conditions. UCNPs provide a dose-dependent and time-dependent cytotoxic effect on all studied cell lines which was most pronounced for the Raw264.7 cell line. It is probably caused by the high phagocytic activity of macrophages. The statistically significant differences in cell viability after 24, 48 and 72 h of incubation of cells with particles were observed just for the macrophage cell line. It is also worth noting that annealed particles are less toxic than unannealed ones.
The study was supported by a grant Russian Science Foundation No. 21-72-10057,
https://rscf.ru/project/21-72-10057/..
File with abstract
Speaker
Irina Yanina
Saratov State University, Tomsk State University
Russia
Report
File with report
Discussion
Ask question
