Development of a set of methods for assessing the biodegradation of copolymer composite materials in vivo
Natalia I. Kazachkina 1, Viktoriya V. Deeva 1, Daria K. Tuchina1,2, Ilya D.Soloviev1, Alexander P. Savitsky 1, Valery V. Tuchin1,2,3, Viktoriya V. Zherdeva 1
1 Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences
2 Saratov State University, Saratov, Russia
3 Saratov State Medical University, Saratov, Russia 4 Institute of Precision Mechanics and Control RAS, Saratov, Russia
Abstract
The aim of our work was to test new hybrid (composite) biocompatible polyester-based copolymers in vitro and in vivo to design implantable devices based on the principle of triboelectric nanogenerators (TENG).
Fluorescence spectroscopy methods were used to evaluate the release of the fluorescent dye indocyanine green (ICG) from labeled polymers. The rate of diffusion of degradation products of copolymers and their markers in solutions and ex vivo was measured by collimated transmission, coherent optical tomography, and reflective diffuse spectroscopy. In addition, the linear dimensions of the copolymer samples were evaluated and the release of the paramagnetic gadolinium-based agent was detected by magnetic resonance imaging as well. In vitro tests on the degradation of copolymers by enzyme hydrolysis were also carried out. The cytotoxicity of the copolymers was evaluated on mouse fibroblast-like, myoblast and neoplasia cell lines.
The set of these approaches will follow by testing of the biodegradation of copolymers in vivo on animals using combined methods of fluorescence imaging and MRI. Thus, It will allow continuous monitoring of the biodegradation of TENG non-invasively in real time.
The work was supported by the grant of Ministry of Science and Higher Education of the Russian Federation no. 13.2251.21.0009 (Agreement no. 075-15-2021-942).
Speaker
Natalia I. Kazachkina
Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences
33, bld. 2 Leninsky Ave., Moscow 119071, Russia (или Russian Federation)
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